草地学报

草地学报 ›› 2021, Vol. 29 ›› Issue (1): 52-59.DOI: 10.11733/j.issn.1007-0435.2021.01.007

• 研究论文 • 上一篇    下一篇

异龄老芒麦根际土壤中脂肪酸类物质检测及根际土壤的自毒效应研究

杨航, 祁娟, 刘文辉, 刘艳君, 宿敬龙, 李明   

  1. 1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中-美草地畜牧业可持续研究中心, 甘肃 兰州 730070;
    2. 青海省畜牧兽医科学院, 青海省青藏高原优良牧草种质资源利用重点实验室, 青海 西宁 810016
  • 收稿日期:2020-08-31 修回日期:2020-10-07 发布日期:2021-01-08
  • 通讯作者: 祁娟
  • 作者简介:杨航(1997-),男,甘肃西和人,硕士研究生,研究方向为草地资源与植物生理生态,E-mail:1468896351@qq.com
  • 基金资助:
    国家自然科学基金（31660684）；国家牧草产业技术体系（CARS-34）；青海省科技厅重点实验室发展专项（2020-ZJ-Y03）共同资助

Fatty Acid Substance Detection and Self-toxic Effect in Rhizosphere Soil of Elymus sibiricus L. with Different Planting Years

YANG Hang, QI Juan, LIU Wen-hui, LIU Yan-jun, SU Jing-long, LI Ming   

  1. 1. College of Grassland Science, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education;China-US Center for Grazing Land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    2. Qinghai Academy of Animal Science and Veterinary Medicine, Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibelan Plateau, Xining 810016, Qinghai Province, China
  • Received:2020-08-31 Revised:2020-10-07 Published:2021-01-08

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摘要： 本研究以3，4，5和8龄老芒麦根际土壤为研究对象，利用气质联用（Gas chromatography-mass spectrometry，GC-MS）技术，分析不同种植年限土壤中脂肪酸酯类化感自毒物质种类及其含量的差异，同时探究不同年限不同浓度（50，100，200，500 g·L-1）老芒麦根际土壤浸提液对其种子萌发及幼苗生长的影响。结果表明：4个不同年限老芒麦根际土壤中共检测出10种脂肪酸酯类物质，且相对含量随种植年限增长呈先升高后降低趋势，其中5龄老芒麦根际土壤中相对含量较高，为50%，而8龄较低，为41%。3龄老芒麦根际土壤中棕榈油酸甲酯较4，5和8龄分别高出98%，62%和58%，5龄土壤中硬脂酸甲酯分别较3，4和8龄高出12%，10%和23%。5和8龄老芒麦根际土壤浸提液对其种子发芽率具有明显的“低促高抑”浓度效应。由双因素方差分析可知，年限对种子发芽率影响极显著，对胚芽长影响较显著。总体来看，随着老芒麦种植年限的增长，土壤中会积累自毒物质，从而导致连作障碍。

关键词: 老芒麦, 自毒效应, 连作障碍, 化感物质, 生长发育

Abstract: The types and contents of fatty acids esters in rhizosphere soil of Elymus sibiricus L. in different planting years (3,4,5 and 8 years) were tested using GC-MS technology. Meanwhile the effects of different concentrations of extracts (50,100,200 and 500 g·L-1) from different planting years rhizosphere soil on seed germination and seedling growth were explored by petri dish. The aim was to provide scientific basis for the cultivation of Elymus sibiricus L. artificial grassland. The results showed that ten kinds of fatty acid substances were detected in different rhizosphere soils. And the relative content of compound of fatty acid component increased first and then decreased gradually with the increase of planting years. The total content of fatty acid lipids was the highest in the 5-years-planted soil and the relative content accounted for 50% and the lowest was 41% in the planted 8 years soil. Among the fatty acid esters in the rhizosphere soil of 3,4,5 and 8 years Elymus sibiricus L.,the methyl palmitoleate in 3-years-planted soil were 98%,62% and 58% higher than thant of 4,5 and 8 years,respectively. However,the methyl stearate content in 5-years-planted soil was 12%,10% and 23% higher than that of 3,4 and 8 years, respectively. Through the effect of soil extract on seed germination and seedling growth of Elymus sibiricus L. research,it was found that the extracts from rhizosphere soil of 5 and 8 years Elymus sibiricus L. had obvious concentration effect of "low promotion and high inhibition" on seed germination rate. According to the two-way ANONA,the years had a significant effect on the germination rate and the germ length. In general,with the increasing of planting years of Elymus sibiricus L. self-toxic substances will accumulate in the soil,which lead to continuous cropping obstacles.

Key words: Elymus sibiricus L., Self-toxic effect, Continuous obstacles, Allelochemicals, Growth and development

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