蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析

doi:10.11733/j.issn.1007-0435.2024.10.002

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3026-3033.DOI: 10.11733/j.issn.1007-0435.2024.10.002

• 研究论文 • 上一篇

蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析

李艳茹¹, 董笛², 晁跃辉¹

1. 北京林业大学/草业与草原学院, 北京 100083;
2. 中国林业科学研究院生态保护与修复研究所, 北京 100091

收稿日期:2024-05-17 修回日期:2024-07-10 发布日期:2024-11-04
通讯作者: 晁跃辉,E-mail:chaoyuehui@bjfu.edu.cn
作者简介:李艳茹(1997-)，女，汉族，山东菏泽人，硕士研究生，主要从事草类植物育种研究，E-mail:liyanru03@163.com
基金资助:
内蒙古草业技术创新中心有限公司项目(CCPTZX2023B04)资助

Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula

LI Yan-ru¹, DONG Di², CHAO Yue-hui¹

1. College of Grassland Industry and Grassland, Beijing Forestry University, Beijing 100083, China;
2. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-05-17 Revised:2024-07-10 Published:2024-11-04

摘要/Abstract

摘要： 叶柄上叶基因(BLADE-ON-PETIOLE,BOP)参与调控植物生长发育中如叶片和花朵的形态形成和脱落等重要发育过程。本研究将蒺藜苜蓿R108作为材料，通过生物信息学分析、亚细胞定位及表达模式分析等实验，探究MtBOP基因的特性。MtBOP基因完整编码区为1452 bp，编码483个氨基酸。根据系统发育进化树结果可知其与豌豆(Pisum sativum)和红三叶草(Trifolium pratense)亲缘关系最近。通过信号肽预测分析，其不具有信号肽。亚细胞定位结果显示MtBOP定位于细胞核和细胞质。表达模式分析表明，该基因的表达量在脱落酸、细胞分裂素、水杨酸、赤霉素调节下有上调趋势，而在生长素，茉莉酸甲酯调节下出现下调趋势。酵母自激活检测显示MtBOP不具有自激活活性。本研究为进一步探究蒺藜苜蓿的MtBOP基因提供了科学依据，而且BOP可用于后续相关基因功能实验。

关键词: 蒺藜苜蓿, MtBOP, 亚细胞定位, 表达分析, 转录自激活

Abstract: BLADE-ON-PETIOLE (BOP) gene is involved in regulating important plant developmental processes such as leaf and flower morphogenesis and shedding. In this study, Medicago truncatula R108 was used as a material to explore the characteristics of MtBOP through bioinformatics analysis,subcellular localization and expression pattern analysis. The complete coding region of MtBOP gene is 1452 bp,encoding 483 amino acids. According to phylogenetic tree results,it is closely related to Pisum sativum and Trifolium pratense. It has no signal peptide according to signal peptide prediction analysis. Subcellular localization results showed that MtBOP was located in the nucleus and cytoplasm. The analysis of expression pattern showed that the expression level of this gene was up-regulated under the regulation of abscisic acid,cytokinin,salicylic acid and gibberellin,while the expression of auxin and methyl jasmonate was down-regulated. The yeast self-activation test showed that MtBOP had no self-activation activity. This study provides scientific basis for further study of MtBOP gene of Medicago truncatula,and BOP can be used for subsequent related protein experiments.

Key words: Medicago truncatula, MtBOP, Subcellular localization, Expression analysis, Transcription from activation

中图分类号:

S541

李艳茹, 董笛, 晁跃辉. 蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析[J]. 草地学报, 2024, 32(10): 3026-3033.

LI Yan-ru, DONG Di, CHAO Yue-hui. Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula[J]. Acta Agrestia Sinica, 2024, 32(10): 3026-3033.

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参考文献

[1] STEEVES T A,SUSSEX I M. Patterns in plant development:embryogenesis:beginnings of development[J]. Biology,1989(10):82031487
[2] HA C M,KIM G T,KIM B C,et al. The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis[J]. Development,2003,130(1):161-172
[3] HEPWORTH S R,ZHANG Y L,MCKIM S,et al. BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis[J]. Plant Cell,2005,17(5):1434-1448
[4] HA C M,JUN J H,NAM H G,et al. BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana[J]. Plant and Cell Physiology,2004,45(10):1361-1370
[5] NORBERG M,HOLMLUND M,NILSSON O. The BLADE ON PETIOLE genes act redundantly to control the growthand development of lateral organs[J]. Development,2005,132(9):2203-2213
[6] LINCOLN C,LONG J,YAMAGUCHI J,et al. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants[J]. The Plant Cell Online,1994,6(12):1859-1876
[7] DOCKX J,QUAEDVLIEG N,KEULTJES G,et al. The homeobox gene ATK1 of Arabidopsis thaliana is expressed in the shoot apex of the seedling and in flowers and inflorescence stems of mature plants[J]. Plant Molecular Biology,1995,28(4):723-737
[8] JACKSON D,VEIT B,HAKE S. Expression of maize KNOTTED1 related homeobox genes in the shoot apical meristem predicts patterns of morphogenesis in the vegetative shoot[J]. Development,1994,120(2):405-413
[9] LONG J A,MEDFORD J I,BARTON M K,et al. A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis[J]. Nature,1996,379(6560):66-69
[10] WAITES R,HUDSON A. PHANTASTICA:a gene required for dorsoventrality of leaves in Antirrhinum majus[J]. Development,1995,121(7):2143-2154
[11] BENKOVÁ E,MICHNIEWICZ M,SAUER M,et al. Local,efflux-dependent auxin gradients as a common module for plant organ formation[J]. Cell,2003,115(5):591-602
[12] JUN J H,HA C M,FLETCHER J C. BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2[J]. Plant Cell,2010,22(1):62-76
[13] MCKIM S M,STENVIK G E,BUTENKO M A,et al. The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis[J]. Development,2008,135(8):1537-1546
[14] 匡倩,宗亚倩,杨文,等. 空间诱变对‘德钦’紫花苜蓿农艺性状和营养品质的影响[J]. 草地学报,2024,32(6):1962-1967
[15] 谢宏,李舒文,董迪,等. 蒺藜苜蓿MtDWF1基因克隆、亚细胞定位及表达特征分析[J]. 草地学报,2023,31(4):984-991
[16] 魏臻武,盖钧镒. 豆模式植物——蒺藜苜蓿[J]. 草业学报,2008(1):114-120
[17] ENDRE G,KERESZT A,KEVEI Z,et al. A receptor kinase gene regulating symbiotic nodule development[J]. Nature,2002,417(6892):962-966
[18] STACEY G,LIBAULT M,BRECHENMACHER L,et al. Genetics and functional genomics of legume nodulation[J]. Current Opinion in Plant Biology,2006,9(2):110-121
[19] 王菊萍,王珍,张铁军,等. 蒺藜苜蓿MtbHLH148转录因子的克隆与转化及其功能分析[J]. 西北植物学报,2019,39(6):963-973
[20] MHAMDI A. NPR1 Has Everything under Control[J]. Plant Physiology,2019,181(1):6-7
[21] CAO H,GLAZEBROOK J,CLARKE J D,et al. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J]. Cell,1997,88(1):57-63
[22] ZHANG Y,FAN W,KINKEMA M,et al. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J]. Proceedings of the National Academy of Sciences,1999,96(11):6523-6528
[23] FAN W,DONG X. In Vivo interaction between NPR1 and transcription factor TGA2 Leads to salicylic acid-mediated gene activation in arabidopsis[J]. Plant Cell,2002,14(6):1377-1389
[24] 常璟. 棉花离层相关基因GhBOP1的分离及功能鉴定[D]. 兰州:甘肃农业大学,2015:44-46(责任编辑刘婷婷)第32卷第10期 Vol.32 No. 10草地学报 ACTAAGRESTIASINICA 2024年 10月

蒺藜苜蓿MtBOP的克隆、亚细胞定位及表达分析

Cloning,Subcellular Localization and Expression Analysis of MtBOP from Medicago truncatula

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