黑果枸杞YABBY转录因子家族鉴定与生物信息学分析

doi:10.11733/j.issn.1007-0435.2024.06.009

草地学报 ›› 2024, Vol. 32 ›› Issue (6): 1729-1741.DOI: 10.11733/j.issn.1007-0435.2024.06.009

• 研究论文 • 上一篇

黑果枸杞YABBY转录因子家族鉴定与生物信息学分析

刘筠¹, 李效雄², 贾西贝¹, 马瑞¹, 柳迪¹, 杜雨¹, 胡晓桐¹, 马彦军¹

1. 甘肃农业大学林学院, 甘肃兰州 730070;
2. 兰州资源环境职业技术大学, 甘肃兰州 730030

收稿日期:2023-11-07 修回日期:2024-01-21 发布日期:2024-06-29
通讯作者: 马彦军,E-mail:mayanjun@gsau.edu.cn
作者简介:刘筠(1995-)，女，汉族，甘肃礼县人，硕士研究生，主要从事林木遗传育种研究，E-mail：1223318211@qq.com
基金资助:
兰州资源环境职业技术大学重点研究项目(X2022ZD-05)；兰州市科技计划项目(2022-2-22)；甘肃省高校产业支撑计划(2023CYZC-46)资助

Identification and Bioinformatics Analysis of YABBY Transcription Factor Family in Lycium ruthenicum

LIU Yun¹, LI Xiao-xiong², JIA Xi-bei¹, Ma Rui¹, LIU Di¹, DU Yu¹, HU Xiao-tong¹, MA Yan-jun¹

1. Forest College, Gansu Agriculture University, Lanzhou, Gansu Province 730070, China;
2. Lan Zhou Resources & Environment Voc-tech University, Lanzhou, Gansu Province 730030, China

Received:2023-11-07 Revised:2024-01-21 Published:2024-06-29

摘要/Abstract

摘要： 为探究黑果枸杞(Lycium ruthenicum)YABBY基因家族在盐胁迫下的耐盐分子机制，本文采用生物信息学方法，对黑果枸杞转录组数据库中YABBY家族成员的理化性质、保守结构域、进化树及表达模式进行分析。结果表明，黑果枸杞转录组数据中共筛选出10个YABBY家族成员，分布在YAB1/YAB3，YAB2和YAB5亚家族中。蛋白质亚细胞定位预测结果表明，其家族成员主要定位于细胞核。黑果枸杞YABBY家族蛋白分子量介于11 676.16~23 751.85 D；等电点介于6.47~9.62；总原子数介于1 584~3 294；氨基酸个数介于106~213 aa；脂溶指数介于55.06~81.04；不稳定系数介于26.38~70.05；蛋白成员均为亲水蛋白。LrYABBY家族的蛋白质C端具有YABBY保守结构域，无规则卷曲和α-螺旋占比较大。表达分析证明，NaCl胁迫株的不同器官中LrYABBYs具有不同的表达趋势，LrYABBY10在根中高表达，LrYABBY8在叶中高表达，表明其可能在黑果枸杞盐胁迫响应中发挥重要作用。该结果为黑果枸杞YABBY家族及其盐胁迫响应方面的进一步研究提供参考。

关键词: 黑果枸杞, NaCl胁迫, YABBY转录因子, 生物信息学分析

Abstract: To investigate the salt tolerance molecular mechanism of the YABBY gene family in Lycium ruthenicum under salt stress,the bioinformatics methods was used to study and analyze the physicochemical properties,conserved domains,evolutionary trees,and expression patterns of YABBY family members in the transcriptome database of Lycium ruthenicum. The results showed that a total of 10 members of the YABBY family genes were screened from the transcriptome data of Lycium ruthenicum,belonging to the YAB1/YAB3,YAB2,and YAB5 subfamilies. The prediction results of protein subcellular localization indicated that its family members are mainly located in the nucleus. The molecular weight of the YABBY family proteins ranged from 11 676.16 to 23 751.85 D. The isoelectric point ranged from 6.47 to 9.62. The total atomic number was between 1 584 and 3 294. The number of amino acids ranged from 106 to 213 aa. The lipid solubility index ranged from 55.06 to 81.04. The instability coefficient ranged from 26.38 to 70.05. All of LrYABBY proteins were hydrophilic proteins. The C-terminal of LrYABBY proteins had YABBY conserved domain,random curling and α-helix occupy a large proportion. Expression analysis had shown that LrYABBYs had different expression trends in different organs of NaCl stressed plants. LrYABBY10 was highly expressed in the roots,while LrYABBY8 was highly expressed in the leaves,indicating that it maight play an important role in the response of Lycium ruthenicum to salt stress. This result provided a reference for further research on the YABBY family genes of Lycium ruthenicum and their salt stress responses.

Key words: Lycium ruthenicum, NaCl treatment, YABBY transcription factor, Bioinformatics analysis

中图分类号:

S722.3+6

刘筠, 李效雄, 贾西贝, 马瑞, 柳迪, 杜雨, 胡晓桐, 马彦军. 黑果枸杞YABBY转录因子家族鉴定与生物信息学分析[J]. 草地学报, 2024, 32(6): 1729-1741.

LIU Yun, LI Xiao-xiong, JIA Xi-bei, Ma Rui, LIU Di, DU Yu, HU Xiao-tong, MA Yan-jun. Identification and Bioinformatics Analysis of YABBY Transcription Factor Family in Lycium ruthenicum[J]. Acta Agrestia Sinica, 2024, 32(6): 1729-1741.

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黑果枸杞YABBY转录因子家族鉴定与生物信息学分析

Identification and Bioinformatics Analysis of YABBY Transcription Factor Family in Lycium ruthenicum

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