关键词: 青海草原毛虫, 离子型受体, 生物信息学分析, 表达谱分析

Abstract: In this study,based on the transcriptome data of Gynaephora qinghaiensis,bioinformatics methods and qRT-PCR technology were used to identify and analyze the expression profiling of GqinIRs genes. In this study,a total of 12 GqinIRs genes were identified,and bioinformatics analysis showed that the IRs of Gynaephora qinghaiensis belonged to amphoteric proteins,except for GqinIR7,GqinIR8a,GqinIR10a,GqinIR75a,and GqinIR76b,all of them had signal peptides and 2~4 transmembrane domains,and the second- and third-order structures were mainly composed of α helix and random coils. Phylogenetic results showed that the IR of Gynaephora qinghaiensis was closely related to Oriental armyworm and Spodoptera exigua. Gene expression profile analysis showed that the relative expression levels of the 12 IR genes in different tissues of Gynaephora qinghaiensis were different,and the GqinIR6 gene was basically not expressed in the antennae of the male,while the remaining 11 GqinIRs genes were expressed in the antennae of the male,while their expression levels were different (P<0.05). This study provides an important basis for further study of the recognition and response mechanism of ionotype receptor proteins of Gynaephora qinghaiensis to external odor molecules,and lays a foundation for further research on the olfactory mechanism of Gynaephora qinghaiensis.

Key words: Gynaephora qinghaiensis, Ionotropic receptors, Bioinformatics analysis, Expression profile analysis