草地学报

›› 2014, Vol. 22 ›› Issue (1): 203-207.DOI: 10.11733/j.issn.1007-0435.2014.01.032

• 研究简报 • 上一篇    下一篇

SRAP标记对8份假俭草材料的鉴定分析

郭海林, 郭爱桂, 宗俊勤, 袁学军, 张芳, 刘建秀   

  1. 江苏省中国科学院植物研究所(南京中山植物园), 江苏 南京 210014
  • 收稿日期:2013-05-30 修回日期:2013-08-05 出版日期:2014-02-15 发布日期:2014-01-28
  • 通讯作者: 刘建秀，E-mail：turfunit@aliyun.com
  • 作者简介:郭海林（1975-），女，内蒙古乌兰察布人，博士，副研究员，主要从事草坪草遗传育种与改良研究，E-mail：ghlnmg@sina.com
  • 基金资助:
    江苏省科技支撑计划项目（BE2008403）；江苏省平台项目（BM2010050）；江苏省水利科技项目（2012053）资助

Identification Analysis of Eight Centipedegrass Materials by SRAP Molecular Markers

GUO Hai-lin, GUO Ai-gui, ZONG Jun-qin, YUAN Xue-jun, ZHANG Fang, LIU Jian-xiu   

  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China
  • Received:2013-05-30 Revised:2013-08-05 Online:2014-02-15 Published:2014-01-28

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摘要： 应用SRAP分子标记技术对6个假俭草（Eremochloa ophiuroides）优良品系和2个引进品种进行扩增扫描，从分子的角度对8份材料进行鉴定。结果表明：31对SRAP引物在8份材料中扩增出576条清晰的谱条，其中多态性条带359个，多态性比率为62.33%，8份材料间的遗传相似系数变异范围为0.6962～0.8177，平均遗传相似系数为0.7388。31对SRAP引物中有1对引物Me18Em17可以将所有材料区分开来，应用该引物的扩增谱带构建了参试材料的DNA指纹图谱，可将不同材料从DNA水平上加以区分。

关键词: 假俭草, 优良品系, 鉴定, SRAP

Abstract: Six excellent strains and two cultivars of centipederass were amplified by 31 SRAP primers. As a result, a total of 576 clear bands were detected and 359 were polymorphic with PPB (Percentage of Polymorphic Bands) at 62.33%. The genetic similarity among eight tested materials ranged from 0.6962 to 0.8177 with the average of 0.7388.One primer of Me18Em17 was selected to construct DNA fingerprint of 8 tested materials. It was able to distinguish each tested cultivar from others by polymorphic bands.

Key words: Centipedegrass, Strains, Identification, SRAP

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